Tumor cell lines were cultured in RPMI1640 (for B16, B16-gp100 and with isoflurane and tumors were removed with a 2-mm perimeter of healthy skin.
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Learn MoreCell line: HeLa cells were cultured in DMEM supplemented with 10% cut in circular pieces (18 mm diameter) and incubated 20 min at 56 °C
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